Osu! Everybody's favorite DS rhythm action game is back! Prepare to help people and save the world again with the power of dance and song in Moero! Nekketsu Rhythm Damashii Osu! Tatakae! Ouendan 2. This is a direct sequel to Osu! Tatakae! Ouendan and not Elite Beat Agents, which is a remake of sorts of the first game with different songs, characters and stories for the western market.

Moero! Nekketsu Rhythm Damashii follows more or less the storyline from the original Osu! Tatakae! Ouendan, and is set roughly four years after the original game. Players act as the leader of a three-person cheerleading squad. Whenever someone is stressed out or backed into a corner, all they need to do is shout "Ouendan!" (Japanese for "Cheer Squad"). Then, the Ouendan appear (usually out of a place like a closet or are there already eating ramen) and use cheering and dance to help the that someone through their troubles.

The original characters (signified by wearing their long Gakuran jackets) are still there, cheering people on. However, a new set of rivals appear that challenge the original Ouendan in skill and cheering (the "new" Ouendan are signified by their blue military-style outfits.) The individual stories are all unrelated, but characters featured in one stage may appear as background or supporting characters in another. In the final stage, all of the characters in the game unite when the two Ouendan join together and once again lead the entire world in a cheer to save Earth.